This Research Assistant position is in the laboratory of Dr. Mary Weber in the Department of Microbiology and Immunology it is needed to assist with cutting-edge research aimed at understanding how Chlamydia trachomatis and Orientia tsutsugamushi secretion system effector proteins subvert host cell processes. This will employ molecular, cellular, biochemical, and genetic techniques.

• Perform library and online research on specified topics for use in experimentation.


• Perform experimental studies based on given hypotheses and goals; evaluate and record outcomes of experiments based on hypotheses and goals and submit for investigator’s inspection.


• Assist in the analysis and interpretation of experiments.


• Observe techniques in other laboratories.


• Maintain and passage multiple cell lines.


• Purify bacteria from infected cells by density gradient.


• Use immunofluorescent microscopy to titer bacterial stocks.


• Perform molecular biology experiments including PCR and western blotting.


• Prepare chemical solutions and media according to established protocols.


• Maintain bacterial and eukaryotic stocks and inventory.


• Complete work within specified time guidelines; discuss progress and any problems as needed.


• Present research progress at lab meetings.


• Assist in the preparation of manuscripts and grants by organizing data and preparing images, figures, and tables as directed.


• Assist in maintaining accurate records of laboratory databases (equipment and supplies, reagents, and microbial strains and stocks).


• Maintain laboratory supplies and equipment, including the timely ordering, receiving, and proper storage of supplies and equipment.


• Assist in maintaining general laboratory functionality.


• Assist in developing new protocols.


• Maintain a clean work area.

A Bachelor’s degree in Microbiology, Molecular Biology, Cell Biology, or Immunology, or an equivalent combination of education and experience.

A minimum of 3-months experience working in a microbiology or molecular biology laboratory is required. Prior experience working with pathogenic microorganisms is preferred, but not required. Excellent written and verbal communication skills.

• Basic proficiency with each of the following:
  
  
  
  
  • General microbiologic techniques, such as culture, storage, and safe handling of pathogenic agents.
  
  
  • Standard molecular techniques, including PCR, plasmid purification, bacterial transformation, agarose gel electrophoresis, and western blotting.
  
  
  • Mammalian cell culture and sterile techniques.
  
  
  • Gathering, recording, and analyzing of experimental data.
  
  
  • Data analysis and presentation software, such as Excel, Photoshop, and GraphPad Prism.
  
  
  
  


• Working proficiency with each of the following:
  
  
  
  • Ability to effectively prioritize projects to consistently meet deadlines.
  
  
  • Ability to multitask in a fast-paced environment.
  
  
  • Attention to detail and accuracy.
  
  
  
  

Highly Desirable

• Working proficiency with each of the following:
  
  
  
  
  • Passaging and maintaining various cell lines.
  
  
  • Immunofluorescence microscopy.